Human sTREM-1 (Triggering Receptor Expressed On Myeloid Cells 1) Antibody Pair (STJAP00235)
General Information
| Short Description | This sTREM-1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | TREM1 |
| Gene ID | 54210 |
| Uniprot ID | TREM1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human sTREM-1 protein.Detection Immunogen: Recombinant Human sTREM-1 protein. |
Additional Info
| Post Translational Modifications | Glycosylated. |
| Function | Isoform 1: Cell surface receptor that plays important roles in innate and adaptive immunity by amplifying inflammatory responses. Upon activation by various ligands such as PGLYRP1, HMGB1 or HSP70, multimerizes and forms a complex with transmembrane adapter TYROBP/DAP12. In turn, initiates a SYK-mediated cascade of tyrosine phosphorylation, activating multiple downstream mediators such as BTK, MAPK1, MAPK3 or phospholipase C-gamma. This cascade promotes the neutrophil- and macrophage-mediated release of pro-inflammatory cytokines and/or chemokines, as well as their migration and thereby amplifies inflammatory responses that are triggered by bacterial and fungal infections. By also promoting the amplification of inflammatory signals that are initially triggered by Toll-like receptor (TLR) and NOD-like receptor engagement, plays a major role in the pathophysiology of acute and chronic inflammatory diseases of different etiologies including septic shock and atherosclerosis. Isoform 2: Acts as a decoy receptor, counterbalancing TREM1 pro-inflammatory activity through the neutralization of its ligand. |
| Protein Name | Triggering Receptor Expressed On Myeloid Cells 1Trem-1Triggering Receptor Expressed On Monocytes 1Cd Antigen Cd354 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-202733Reactome: R-HSA-2172127 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinRecruited To Lipid Rafts When ActivatedIsoform 2: Secreted |
| Alternative Antibody Pair Names | Triggering Receptor Expressed On Myeloid Cells 1 antibody pairTrem-1 antibody pairTriggering Receptor Expressed On Monocytes 1 antibody pairCd Antigen Cd354 antibody pairTREM1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human sTREM-1 (Triggering Receptor Expressed On Myeloid Cells 1) Antibody Pair (STJAP00235)
This antibody pair detects soluble TREM-1 (Triggering Receptor Expressed On Myeloid Cells 1) in human samples only. The UniProt accession for the target is Q9NP99.
The capture antibody is supplied unconjugated at 0.5 mg/mL, while the detection antibody is supplied biotinylated at 0.5 mg/mL. Both are polyclonal antibodies raised in goat and formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair has been validated for use in ELISA only. No other assay formats are listed in the product documentation.
Both the capture and detection antibodies were raised against recombinant human sTREM-1 protein. Both antibodies were subsequently purified by antigen affinity chromatography.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.