| Short Description : | Recombinant-Human STAT1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications: | ELISA/Immunogen/SDS-PAGE/WB |
| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Dilution Range: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | STAT1 |
| Gene ID: | 6772 |
| Uniprot ID: | STAT1_HUMAN |
| Immunogen: | Homo sapiens (Human) |
| Immunogen Region: | Met1-Arg683 |
| Function | Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6. Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription. Involved in food tolerance in small intestine: associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine. |
| Protein Name | Signal Transducer And Activator Of Transcription 1-Alpha/BetaTranscription Factor Isgf-3 Components P91/P84 |
| Database Links | Reactome: R-HSA-1059683Reactome: R-HSA-1169408Reactome: R-HSA-1433557Reactome: R-HSA-1839117Reactome: R-HSA-186763Reactome: R-HSA-2173795 P42224-1Reactome: R-HSA-6785807Reactome: R-HSA-877300 P42224-1Reactome: R-HSA-877312 P42224-1Reactome: R-HSA-8854691Reactome: R-HSA-8939902Reactome: R-HSA-8984722Reactome: R-HSA-8985947Reactome: R-HSA-9013508Reactome: R-HSA-9020956Reactome: R-HSA-9020958Reactome: R-HSA-909733Reactome: R-HSA-912694Reactome: R-HSA-9670439Reactome: R-HSA-9673767Reactome: R-HSA-9673770Reactome: R-HSA-9674555Reactome: R-HSA-9680350Reactome: R-HSA-9705462Reactome: R-HSA-9705671Reactome: R-HSA-9725370Reactome: R-HSA-982772Reactome: R-HSA-9833482Reactome: R-HSA-9860927 |
| Cellular Localisation | CytoplasmNucleusTranslocated Into The Nucleus Upon Tyrosine Phosphorylation And DimerizationIn Response To Ifn-Gamma And Signaling By Activated Fgfr1Fgfr2Fgfr3 Or Fgfr4Monomethylation At Lys-525 Is Required For Phosphorylation At Tyr-701 And Translocation Into The NucleusTranslocates Into The Nucleus In Response To Interferon-Beta Stimulation |
| Alternative Protein Names | Signal Transducer And Activator Of Transcription 1-Alpha/Beta proteinTranscription Factor Isgf-3 Components P91/P84 proteinSTAT1 protein |
Information sourced from Uniprot.org
