Human SPHK1 (Sphingosine Kinase 1) Sandwich ELISA Kit (STJE0012306)
SPECIFICATIONS
General Information
| Short Description | This SPHK1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.114ng/mL |
| Detection Limit | 0.312-20ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | SPHK1 |
| Gene ID | 8877 |
| Uniprot ID | SPHK1_HUMAN |
| Sample Type | tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level). |
| Post Translational Modifications | |
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA. Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. |
| Protein Name | Sphingosine Kinase 1Sk 1Spk 1Acetyltransferase Sphk1 |
| Database Links | Reactome: R-HSA-1660661Reactome: R-HSA-390471Reactome: R-HSA-5218921Reactome: R-HSA-9009391Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmNucleusCell MembraneEndosome MembranePeripheral Membrane ProteinMembraneClathrin-Coated PitSynapseTranslocated From The Cytoplasm To The Plasma Membrane In A Cib1-Dependent MannerBinds To Membranes Containing Negatively Charged Lipids But Not Neutral LipidsRecruited To Endocytic Membranes By Sphingosine Where Promotes Membrane Fusion |
| Alternative ELISA Names | Sphingosine Kinase 1 ELISA kitSk 1 ELISA kitSpk 1 ELISA kitAcetyltransferase Sphk1 ELISA kitSPHK1 ELISA kitSK1 ELISA kitSPHK ELISA kitSPK ELISA kit |
| output |
Information sourced from Uniprot.org