Human SPARC (SPARC) Antibody Pair (STJAP00915)
General Information
| Short Description | This SPARC antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R06-6W-4/R08-6J-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | SPARC |
| Gene ID | 6678 |
| Uniprot ID | SPRC_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Function | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites.an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. |
| Protein Name | SparcBasement-Membrane Protein 40Bm-40OsteonectinOnSecreted Protein Acidic And Rich In Cysteine |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1251985Reactome: R-HSA-3000178Reactome: R-HSA-3000497 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneIn Or Around The Basement Membrane |
| Alternative Antibody Pair Names | Sparc antibody pairBasement-Membrane Protein 40 antibody pairBm-40 antibody pairOsteonectin antibody pairOn antibody pairSecreted Protein Acidic And Rich In Cysteine antibody pairSPARC antibody pairON antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human SPARC (SPARC) Antibody Pair (STJAP00915)
This antibody pair is designed to detect human SPARC (UniProt P09486) and has confirmed reactivity against human samples. No cross-reactivity data for other species is provided in the product specifications.
Both antibodies in the pair are rabbit-derived monoclonal IgGs, with clone IDs R06-6W-4 and R08-6J-5. Both are supplied unconjugated and purified by affinity purification.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both antibodies are formulated in 0.01M PBS at pH 7.2. No carrier proteins, preservatives, or stabilizers are specified beyond this buffer composition.
Working concentrations and dilutions are determined by the end user and are not defined in the product specifications. Titration experiments are recommended to establish optimal conditions for each specific assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.