Human SP-D (Pulmonary Surfactant-Associated Protein D) Antibody Pair (STJAP00238)
General Information
| Short Description | This SP-D antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | SFTPD |
| Gene ID | 6441 |
| Uniprot ID | SFTPD_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human SP-D protein.Detection Immunogen: Recombinant Human SP-D protein. |
Additional Info
| Post Translational Modifications | The N-terminus is blocked. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. S-nitrosylation at Cys-35 and Cys-40 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages. |
| Function | Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. |
| Protein Name | Pulmonary Surfactant-Associated Protein DPsp-DSp-DCollectin-7Lung Surfactant Protein D |
| Database Links | Reactome: R-HSA-166016Reactome: R-HSA-168179Reactome: R-HSA-198933Reactome: R-HSA-391160Reactome: R-HSA-5683826Reactome: R-HSA-5686938Reactome: R-HSA-5688849Reactome: R-HSA-5688890Reactome: R-HSA-9692916Reactome: R-HSA-9705671 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixSurface Film |
| Alternative Antibody Pair Names | Pulmonary Surfactant-Associated Protein D antibody pairPsp-D antibody pairSp-D antibody pairCollectin-7 antibody pairLung Surfactant Protein D antibody pairSFTPD antibody pairCOLEC7 antibody pairPSPD antibody pairSFTP4 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human SP-D (Pulmonary Surfactant-Associated Protein D) Antibody Pair (STJAP00238)
This antibody pair is designed to detect Human SP-D (Pulmonary Surfactant-Associated Protein D), corresponding to UniProt entry P35247. Reactivity has been confirmed for human samples only.
The capture antibody is an unconjugated rabbit polyclonal antibody, and the detection antibody is a biotin-conjugated mouse monoclonal antibody. Both are supplied at 0.5 mg/mL.
Both the capture and detection antibodies are formulated in PBS containing 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are specified in the product documentation.
Both the capture and detection antibodies were raised against recombinant Human SP-D protein. The purification methods used were Protein A for the capture antibody and Protein A or G for the detection antibody.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.