• The following antibodies were used for cell surface molecules expression analysis: CD11c APC, CD80 FITC, CD83 FITC, CD40 PE, CD86 PE, MHC II (HLA-DR) FITC, MHC I (HLA-ABC) FITC, CD1a FITC, CD14 FITC, ILT3 PE, PD-L1 PE, TLR-2 PE, CCR5 PE, CCR7 PE, CXCR1 FITC, CXCR2 PE, CXCR4 PE, CD4 PE, CD4 PeCy7, IFN Gamma APC, all from eBioscience; CCR2 PerCP-Cy5. 5 from BioLegend (San Diego, CA, USA) and CCR1 PE from R&D Systems (Minneapolis, MN, USA). Cells were resuspended in PBS + 10% FBS and incubated with antibodies for 45 minutes, then washed twice with PBS + 3% FBS and fixed with IC Fixation Buffer (eBioscience). GILZ PE antibody (eBioscience) was used for intracellular staining. Cells were fixed, resuspended in Permeabilization Buffer (eBioscience) and incubated with the antibody for 20 minutes, then washed twice with Permeabilization Buffer and resuspended in flow cytometry buffer for data collection. For IFN Gamma intracellular detection cells were stimulated with phorbol-12-myristate-13-acetate (PMA) (50 ng/ml) / ionomycin…
  • The following antibodies were used for cell surface molecules expression analysis: CD11c APC, CD80 FITC, CD83 FITC, CD40 PE, CD86 PE, MHC II (HLA-DR) FITC, MHC I (HLA-ABC) FITC, CD1a FITC, CD14 FITC, ILT3 PE, PD-L1 PE, TLR-2 PE, CCR5 PE, CCR7 PE, CXCR1 FITC, CXCR2 PE, CXCR4 PE, CD4 PE, CD4 PeCy7, IFN Gamma APC, all from eBioscience; CCR2 PerCP-Cy5. 5 from BioLegend (San Diego, CA, USA) and CCR1 PE from R&D Systems (Minneapolis, MN, USA). Cells were resuspended in PBS + 10% FBS and incubated with antibodies for 45 minutes, then washed twice with PBS + 3% FBS and fixed with IC Fixation Buffer (eBioscience). GILZ PE antibody (eBioscience) was used for intracellular staining. Cells were fixed, resuspended in Permeabilization Buffer (eBioscience) and incubated with the antibody for 20 minutes, then washed twice with Permeabilization Buffer and resuspended in flow cytometry buffer for data collection. For IFN Gamma intracellular detection cells were stimulated with phorbol-12-myristate-13-acetate (PMA) (50 ng/ml) / ionomycin…
  • Purified CD4+ (a and b) or CD4+CD25+ T cells (c and d) were cultured alone or with mTECs for 3 days.

Human Soluble CD4 protein (Recombinant) (STJP000334)

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STJP000334

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Soluble CD4-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered solution inPBS, pH7.4.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA
Immunoreactivity: The activity was tested by immobilized recombinant CD4 (1ug/ml) ability to support adhesion of HeLa cells (5x104 cells/well ) ,-80% will adhere aftermin atC. NA
Gene Symbol: CD4
Gene ID: 920
Uniprot ID: CD4_HUMAN
Immunogen Region: ECD
Immunogen: Optimized DNA sequence encoding Human CD4 extracellular domain was expressed in HEK293 cells. NA
Immunogen Sequence: MNRGVPFRHL LLVLQLALLP AATQGKKVVL GKKGDTVELT CTASQKKSIQ FHWKNSNQIK ILGNQGSFLT KGPSKLNDRA DSRRSLWDQG NFPLIIKNLK IEDSDTYICE VEDQKEEVQL LVFGLTANSD THLLQGQSLT LTLESPPGSS PSVQCRSPRG KNIQGGKTLS VSQLELQDSG TWTCTVLQNQ KKVEFKIDIV VLAFQKASSI VYKKEGEQVE FSFPLAFT
Tissue Specificity Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.
Post Translational Modifications Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC.phosphorylation at Ser-433 plays an important role for CD4 internalization.
Function Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1). Down-regulated by HIV-1 Vpu. Acts as a receptor for Human Herpes virus 7/HHV-7.
Protein Name T-Cell Surface Glycoprotein Cd4
T-Cell Surface Antigen T4/Leu-3
Cd Antigen Cd4
Database Links Reactome: R-HSA-1462054
Reactome: R-HSA-167590
Reactome: R-HSA-173107
Reactome: R-HSA-180534
Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-389948
Reactome: R-HSA-449836
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Localizes To Lipid Rafts
Removed From Plasma Membrane By Hiv-1 Nef Protein That Increases Clathrin-Dependent Endocytosis Of This Antigen To Target It To Lysosomal Degradation
Cell Surface Expression Is Also Down-Modulated By Hiv-1 Envelope Polyprotein Gp160 That Interacts With
And Sequesters Cd4 In The Endoplasmic Reticulum
Alternative Protein Names T-Cell Surface Glycoprotein Cd4 protein
T-Cell Surface Antigen T4/Leu-3 protein
Cd Antigen Cd4 protein
CD4 protein

Information sourced from Uniprot.org

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