Human SIGLEC8 (Sialic acid-binding Ig-like lectin 8) CLIA Kit for Quantitative Detection

Name: Human SIGLEC8 (Sialic acid-binding Ig-like lectin 8) CLIA Kit for Quantitative Detection
SKU: STJC0000610
Availability: InStock

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SPECIFICATIONS

STJC0000610

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Short Description	This human SIGLEC8 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	37.5pg/mL
Detection Limit	62.5~4000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	SIGLEC8
Gene ID	27181
Uniprot ID	SIGL8_HUMAN
Specificity	This kit recognizes Human SIGLEC8 in samples. No significant cross-reactivity or interference between Human SIGLEC8 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Expressed specifically on blood cells namely basophil, mast cells and eosinophils.
Function	Putative adhesion molecule that mediates sialic-acid dependent binding to blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.
Protein Name	Sialic Acid-Binding Ig-Like Lectin 8 Siglec-8 Sialoadhesin Family Member 2 Saf-2
Database Links	Reactome: R-HSA-198933
Cellular Localisation	Membrane Single-Pass Type I Membrane Protein
Alternative CLIA Names	Sialic Acid-Binding Ig-Like Lectin 8 CLIA kit Siglec-8 CLIA kit Sialoadhesin Family Member 2 CLIA kit Saf-2 CLIA kit SIGLEC8 CLIA kit SAF2 CLIA kit
Specificity	This kit recognizes Human SIGLEC8 in samples. No significant cross-reactivity or interference between Human SIGLEC8 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	185.11	414.94	1622.13	190.03	452.56	1515.54
Standard deviation	16.40	46.31	98.95	21.80	43.67	104.42
CV (%)	8.86	11.16	6.10	11.47	9.65	6.89