Human SIGLEC3 (Myeloid Cell Surface Antigen Cd33) Antibody Pair (STJAP00458)
General Information
| Short Description | This SIGLEC3 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | CD33 |
| Gene ID | 945 |
| Uniprot ID | CD33_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human SIGLEC3 protien.Detection Immunogen: Recombinant Human SIGLEC3 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent. |
| Function | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K. |
| Protein Name | Myeloid Cell Surface Antigen Cd33Sialic Acid-Binding Ig-Like Lectin 3Siglec-3Gp67Cd Antigen Cd33 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform Cd33m: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Cd33m: PeroxisomeCd33m Isoform Does Not Localize To Cell Surfaces But Instead Accumulates In Peroxisomes |
| Alternative Antibody Pair Names | Myeloid Cell Surface Antigen Cd33 antibody pairSialic Acid-Binding Ig-Like Lectin 3 antibody pairSiglec-3 antibody pairGp67 antibody pairCd Antigen Cd33 antibody pairCD33 antibody pairSIGLEC3 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human SIGLEC3 (Myeloid Cell Surface Antigen Cd33) Antibody Pair (STJAP00458)
This antibody pair detects human SIGLEC3, also known as Myeloid Cell Surface Antigen CD33 (UniProt P20138). Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated, while the detection antibody is biotin-conjugated. Both are rabbit-raised, antigen affinity-purified antibodies supplied at 0.5 mg/mL.
This pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
This pair is suitable for use with serum, plasma, and other biological fluids. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.