Human Siglec-3/CD33 protein (Recombinant) (C-hFc) (STJP019989)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Siglec-3/CD33 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp18-His259) of Human Siglec-3/CD33 (Accession #P20138-1) fused with a C-hFc tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Siglec-3/CD33-C-hFc protein was developed in hek293 cells using the region Asp18-His259. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CD33 |
| Gene ID | 945 |
| Uniprot ID | CD33_HUMAN |
| Immunogen | Recombinant Human Siglec-3/CD33 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp18-His259) of Human Siglec-3/CD33 (Accession #P20138-1) fused with a C-hFc tag at the C-terminus. |
| Immunogen Region | Asp18-His259 |
| Immunogen Sequence | DPNFWLQVQESVTVQEGLCV LVPCTFFHPIPYYDKNSPVH GYWFREGAIISRDSPVATNK LDQEVQEETQGRFRLLGDPS RNNCSLSIVDARRRDNGSYF FRMERGSTKYSYKSPQLSVH VTDLTHRPKILIPGTLEPGH SKNLTCSVSWACEQGTPPIF SWLSAAPTSLGPRTTHSSVL IITPRPQDHGTNLTCQVKFA GAGVTTERTIQLNVTYVPQN PTTGIFPGDGSGKQETRAG |
Additional Info
| Tissue Specificity | Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells. |
| Post Translational Modifications | Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent. |
| Function | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K. |
| Protein Name | Myeloid Cell Surface Antigen Cd33Sialic Acid-Binding Ig-Like Lectin 3Siglec-3Gp67Cd Antigen Cd33 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform Cd33m: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Cd33m: PeroxisomeCd33m Isoform Does Not Localize To Cell Surfaces But Instead Accumulates In Peroxisomes |
| Alternative Protein Names | Myeloid Cell Surface Antigen Cd33 proteinSialic Acid-Binding Ig-Like Lectin 3 proteinSiglec-3 proteinGp67 proteinCd Antigen Cd33 proteinCD33 proteinSIGLEC3 protein |
Information sourced from Uniprot.org