• Biotinylated Human Siglec-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..
  • The purity of Biotinylated Human Siglec-2 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-Siglec-2 Antibody, hFc Tag at 0. 5 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human Siglec-2, His Tag with the EC50 of 19. 1ng/ml determined by ELISA.

Human Siglec-2/CD22 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001622)

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STJP001622

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Siglec-2/CD22-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: CD22
Gene ID: 933
Uniprot ID: CD22_HUMAN
Immunogen Region: Asp20-Arg687
Immunogen: DNA sequence encoding Human Siglec-2/CD22 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2. Phosphorylated on tyrosine residues by LYN.
Function Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow. Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration. Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling. If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced.
Protein Name B-Cell Receptor Cd22
B-Lymphocyte Cell Adhesion Molecule
Bl-Cam
Sialic Acid-Binding Ig-Like Lectin 2
Siglec-2
T-Cell Surface Antigen Leu-14
Cd Antigen Cd22
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-5690714
Reactome: R-HSA-983695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names B-Cell Receptor Cd22 protein
B-Lymphocyte Cell Adhesion Molecule protein
Bl-Cam protein
Sialic Acid-Binding Ig-Like Lectin 2 protein
Siglec-2 protein
T-Cell Surface Antigen Leu-14 protein
Cd Antigen Cd22 protein
CD22 protein
SIGLEC2 protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance