• Biotinylated Human Siglec-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..
  • The purity of Biotinylated Human Siglec-2 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-Siglec-2 Antibody, hFc Tag at 0. 5 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human Siglec-2, His Tag with the EC50 of 19. 1ng/ml determined by ELISA.

Human Siglec-2/CD22 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001622)

SKU:
STJP001622

€773.13 - €2,386.88
Shipping:
Free Shipping
Current Stock:
Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Siglec-2/CD22-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: CD22
Gene ID: 933
Uniprot ID: CD22_HUMAN
Immunogen Region: Asp20-Arg687
Immunogen: DNA sequence encoding Human Siglec-2/CD22 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2. Phosphorylated on tyrosine residues by LYN.
Function Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins.one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
Protein Name B-Cell Receptor Cd22
B-Lymphocyte Cell Adhesion Molecule
Bl-Cam
Sialic Acid-Binding Ig-Like Lectin 2
Siglec-2
T-Cell Surface Antigen Leu-14
Cd Antigen Cd22
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-5690714
Reactome: R-HSA-983695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names B-Cell Receptor Cd22 protein
B-Lymphocyte Cell Adhesion Molecule protein
Bl-Cam protein
Sialic Acid-Binding Ig-Like Lectin 2 protein
Siglec-2 protein
T-Cell Surface Antigen Leu-14 protein
Cd Antigen Cd22 protein
CD22 protein
SIGLEC2 protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance