Human SFN (SFN) Antibody Pair (STJAP00850)
General Information
| Short Description | This SFN antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R01-8I-4/R04-4T-1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4Β°C short term. Aliquot and store at-20Β°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | SFN |
| Gene ID | 2810 |
| Uniprot ID | 1433S_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Present mainly in tissues enriched in stratified squamous keratinizing epithelium. |
| Post Translational Modifications | Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response. Also acts as a TP53/p53-regulated inhibitor of G2/M progression. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis. Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. |
| Protein Name | 14-3-3 Protein SigmaEpithelial Cell Marker Protein 1Stratifin |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-1445148Reactome: R-HSA-5625740Reactome: R-HSA-5628897Reactome: R-HSA-6804114Reactome: R-HSA-75035Reactome: R-HSA-9614399Reactome: R-HSA-9735871Reactome: R-HSA-9755779 |
| Cellular Localisation | CytoplasmNucleusSecretedMay Be Secreted By A Non-Classical Secretory Pathway |
| Alternative Antibody Pair Names | 14-3-3 Protein Sigma antibody pairEpithelial Cell Marker Protein 1 antibody pairStratifin antibody pairSFN antibody pairHME1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human SFN (SFN) Antibody Pair (STJAP00850)
This antibody pair detects human SFN (Stratifin, also known as 14-3-3 sigma), corresponding to UniProt entry P31947. Reactivity has been confirmed for human samples only.
Both antibodies in the pair are rabbit monoclonal IgGs. The clone IDs are R01-8I-4 and R04-4T-1, and both were raised against a recombinant protein immunogen.
This antibody pair has been validated for use in ELISA. It is intended for research use only and has not been validated for diagnostic or therapeutic applications.
Both antibodies are supplied unconjugated and are formulated in 0.01M PBS at pH 7.2. They have been purified by affinity purification.
Working concentrations and dilution ranges are not pre-specified and must be determined by the end user through titration in their specific experimental system.
Both antibodies should be stored at 4Β°C for short-term use. For long-term storage, aliquot and store at -20Β°C. Repeated freeze/thaw cycles should be avoided.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.