Human SEMA4D (Semaphorin-4D) Sandwich ELISA Kit (STJE0003564)

Name: Human SEMA4D (Semaphorin-4D) Sandwich ELISA Kit (STJE0003564)
SKU: STJE0003564
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0003564

Current Stock:

🚚 Free UK Delivery on orders over £150

Quantity:

Processing The item has been added

Enquire For Bulk Order

✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

Short Description	This SEMA4D Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.19ng/mL
Detection Limit	0.31~20ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	SEMA4D
Gene ID	10507
Uniprot ID	SEM4D_HUMAN
Specificity	This kit recognizes Human SEMA4D in samples. No significant cross-reactivity or interference between Human SEMA4D and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system.induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.
Protein Name	Semaphorin-4d A8 Bb18 Gr3 Cd Antigen Cd100
Database Links	Reactome: R-HSA-416550 Reactome: R-HSA-416572 Reactome: R-HSA-416700
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein
Alternative ELISA Names	Semaphorin-4d ELISA kit A8 ELISA kit Bb18 ELISA kit Gr3 ELISA kit Cd Antigen Cd100 ELISA kit SEMA4D ELISA kit C9orf164 ELISA kit CD100 ELISA kit SEMAJ ELISA kit
Specificity	This kit recognizes Human SEMA4D in samples. No significant cross-reactivity or interference between Human SEMA4D and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.05	2.48	9.92	0.96	2.31	10.06
Standard deviation	0.07	0.14	0.46	0.06	0.10	0.45
CV (%)	6.67	5.65	4.64	6.25	4.33	4.47