Human SEMA4D (Semaphorin-4d) Antibody Pair (STJAP00415)
General Information
| Short Description | This SEMA4D antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | SEMA4D |
| Gene ID | 10507 |
| Uniprot ID | SEM4D_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human SEMA4D protien.Detection Immunogen: Recombinant Human SEMA4D protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system.induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. |
| Protein Name | Semaphorin-4dA8Bb18Gr3Cd Antigen Cd100 |
| Database Links | Reactome: R-HSA-416550Reactome: R-HSA-416572Reactome: R-HSA-416700 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Pair Names | Semaphorin-4d antibody pairA8 antibody pairBb18 antibody pairGr3 antibody pairCd Antigen Cd100 antibody pairSEMA4D antibody pairC9orf164 antibody pairCD100 antibody pairSEMAJ antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human SEMA4D (Semaphorin-4d) Antibody Pair (STJAP00415)
This antibody pair detects human SEMA4D (Semaphorin-4D, UniProt Q92854) and is validated for human reactivity only. It is not confirmed for use with samples from other species.
The capture antibody is unconjugated and rabbit-hosted, purified by antigen affinity, and supplied in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is biotin-conjugated and rat-hosted, purified by Protein A or G, and supplied in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components are provided at 0.5 mg/mL.
This antibody pair is validated for use in ELISA. No other assay formats are confirmed for this product.
This antibody pair is suitable for use with serum, plasma, and other biological fluids. Suitability for additional sample types such as cell lysates or tissue homogenates has not been confirmed in the provided product details.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity and performance.
Both the capture and detection antibodies were raised against recombinant human SEMA4D protein. No immunogen region information is specified for either component.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.