Human SCFR (SCFR) Antibody Pair (STJAP00976)
General Information
| Short Description | This SCFR antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL/0.5-4ug/mL |
| Dilution Range | 1:1000-1:10000 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Detection | Colorimetric method;ELISA;Sandwich |
Target Information
| Gene Symbol | KIT |
| Gene ID | 3815 |
| Uniprot ID | KIT_HUMAN |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation. Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. |
| Protein Name | Mast/Stem Cell Growth Factor Receptor KitScfrPiebald Trait ProteinPbtProto-Oncogene C-KitTyrosine-Protein Kinase KitP145 C-KitV-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene HomologCd Antigen Cd117 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-1433557Reactome: R-HSA-1433559Reactome: R-HSA-2219530Reactome: R-HSA-5673001Reactome: R-HSA-6811558Reactome: R-HSA-8866910Reactome: R-HSA-9669914Reactome: R-HSA-9669917Reactome: R-HSA-9669921Reactome: R-HSA-9669924Reactome: R-HSA-9669926Reactome: R-HSA-9669929Reactome: R-HSA-9669933Reactome: R-HSA-9669934Reactome: R-HSA-9669935Reactome: R-HSA-9669936Reactome: R-HSA-9670439Reactome: R-HSA-9680187Reactome: R-HSA-9856649 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Cell MembraneIsoform 3: CytoplasmDetected In The Cytoplasm Of SpermatozoaEspecially In The Equatorial And Subacrosomal Region Of The Sperm Head |
| Alternative Antibody Pair Names | Mast/Stem Cell Growth Factor Receptor Kit antibody pairScfr antibody pairPiebald Trait Protein antibody pairPbt antibody pairProto-Oncogene C-Kit antibody pairTyrosine-Protein Kinase Kit antibody pairP145 C-Kit antibody pairV-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog antibody pairCd Antigen Cd117 antibody pairKIT antibody pairSCFR antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human SCFR (SCFR) Antibody Pair (STJAP00976)
This antibody pair detects human SCFR (also known as CD117 or c-Kit, UniProt P10721) and is validated for reactivity with human samples only.
This antibody pair has been validated for use with serum, plasma, and other biological fluids.
Both antibodies are raised in goat. The capture antibody is supplied unconjugated at 0.5 mg/mL, and the detection antibody is biotinylated at a concentration of 0.5 to 4 ug/mL.
This antibody pair is validated for sandwich ELISA using a colorimetric detection method. It is not validated for other assay formats such as Western blot or flow cytometry.
A dilution range of 1:1000 to 1:10000 is recommended, though optimal dilutions should be determined empirically for each specific experimental setup.
Both components should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.