Human SCF (Kit Ligand) Antibody Pair (STJAP00218)
General Information
| Short Description | This SCF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A.Detection Purification: Protein A. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | KITLG |
| Gene ID | 4254 |
| Uniprot ID | SCF_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human SCF protein.Detection Immunogen: Recombinant Human SCF protein. |
Additional Info
| Function | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
| Protein Name | Kit LigandMast Cell Growth FactorMgfStem Cell FactorScfC-Kit Ligand Cleaved Into - Soluble Kit LigandSkitlg |
| Database Links | Reactome: R-HSA-1257604 P21583-1Reactome: R-HSA-1433557 P21583-1Reactome: R-HSA-1433559 P21583-1Reactome: R-HSA-2219530 P21583-1Reactome: R-HSA-5673001 P21583-1Reactome: R-HSA-6811558 P21583-1Reactome: R-HSA-9856649 P21583-1 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: CytoplasmCytoplasmCytoskeletonCell MembraneCell ProjectionLamellipodiumFilopodiumSoluble Kit Ligand: Secreted |
| Alternative Antibody Pair Names | Kit Ligand antibody pairMast Cell Growth Factor antibody pairMgf antibody pairStem Cell Factor antibody pairScf antibody pairC-Kit Ligand Cleaved Into - Soluble Kit Ligand antibody pairSkitlg antibody pairKITLG antibody pairMGF antibody pairSCF antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human SCF (Kit Ligand) Antibody Pair (STJAP00218)
This antibody pair detects human SCF (Stem Cell Factor, also known as Kit Ligand), corresponding to UniProt entry P21583. Reactivity is confirmed for human only.
The capture antibody is supplied unconjugated at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol, pH 7.4. The detection antibody is biotinylated and supplied at the same concentration and formulation.
This antibody pair has been validated for use in ELISA. No other assay formats are specified in the product details.
Both the capture and detection antibodies are rabbit-derived monoclonal antibodies. Both were raised against recombinant human SCF protein.
Both the capture and detection antibodies were purified using Protein A affinity chromatography.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.