Human sCD40L (Cd40 Ligand) Antibody Pair (STJAP00162)
General Information
| Short Description | This sCD40L antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | CD40LG |
| Gene ID | 959 |
| Uniprot ID | CD40L_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human sCD40L protein.Detection Immunogen: Recombinant Human sCD40L protein. |
Additional Info
| Post Translational Modifications | The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. |
| Function | Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. CD40 ligand, soluble form: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3.both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling. |
| Protein Name | Cd40 LigandCd40-LT-Cell Antigen Gp39Tnf-Related Activation ProteinTrapTumor Necrosis Factor Ligand Superfamily Member 5Cd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble FormScd40l |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-5668541Reactome: R-HSA-5676594 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinCell SurfaceCd40 LigandSoluble Form: SecretedRelease Of Soluble Cd40l From Platelets Is Partially Regulated By Gp Iib/IiiaActin PolymerizationAnd A Matrix Metalloproteinases (Mmp) Inhibitor-Sensitive Pathway |
| Alternative Antibody Pair Names | Cd40 Ligand antibody pairCd40-L antibody pairT-Cell Antigen Gp39 antibody pairTnf-Related Activation Protein antibody pairTrap antibody pairTumor Necrosis Factor Ligand Superfamily Member 5 antibody pairCd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble Form antibody pairScd40l antibody pairCD40LG antibody pairCD40L antibody pairTNFSF5 antibody pairTRAP antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human sCD40L (Cd40 Ligand) Antibody Pair (STJAP00162)
This antibody pair detects human soluble CD40 Ligand (sCD40L, UniProt P29965) and has confirmed reactivity with human samples only. Both the capture and detection antibodies were raised against recombinant human sCD40L protein.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
The capture antibody is supplied unconjugated and the detection antibody is supplied biotinylated, both at 0.5 mg/mL. Each component is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4, and both were purified by Protein A or G affinity chromatography.
Both the capture and detection antibodies are monoclonal and were raised in mouse. Clone IDs and isotypes are not specified in the current product details.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.