Human sCD14 (Monocyte Differentiation Antigen Cd14) Antibody Pair (STJAP00057)
General Information
| Short Description | This sCD14 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Sheep |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | CD14 |
| Gene ID | 929 |
| Uniprot ID | CD14_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human sCD14 protein.Detection Immunogen: Recombinant Human sCD14 protein. |
Additional Info
| Post Translational Modifications | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
| Function | Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). |
| Protein Name | Monocyte Differentiation Antigen Cd14My23 AntigenMyeloid Cell-Specific Leucine-Rich GlycoproteinCd Antigen Cd14 Cleaved Into - Monocyte Differentiation Antigen Cd14 - Urinary Form - Monocyte Differentiation Antigen Cd14 - Membrane-Bound Form |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-140534Reactome: R-HSA-166016Reactome: R-HSA-166020Reactome: R-HSA-166058Reactome: R-HSA-166166Reactome: R-HSA-168179Reactome: R-HSA-168188Reactome: R-HSA-2562578Reactome: R-HSA-5602498Reactome: R-HSA-5603041Reactome: R-HSA-5686938Reactome: R-HSA-6798695Reactome: R-HSA-936964Reactome: R-HSA-937041Reactome: R-HSA-937072Reactome: R-HSA-975163Reactome: R-HSA-9820960Reactome: R-HSA-9824878Reactome: R-HSA-9833110 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorSecretedMembrane RaftGolgi ApparatusSecreted Forms May Arise By Cleavage Of The Gpi Anchor |
| Alternative Antibody Pair Names | Monocyte Differentiation Antigen Cd14 antibody pairMy23 Antigen antibody pairMyeloid Cell-Specific Leucine-Rich Glycoprotein antibody pairCd Antigen Cd14 Cleaved Into - Monocyte Differentiation Antigen Cd14 - Urinary Form - Monocyte Differentiation Antigen Cd14 - Membrane-Bound Form antibody pairCD14 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human sCD14 (Monocyte Differentiation Antigen Cd14) Antibody Pair (STJAP00057)
This antibody pair detects human soluble CD14 (sCD14, Monocyte Differentiation Antigen CD14, UniProt P08571) and is validated for reactivity in human samples only.
The capture antibody is a mouse monoclonal antibody supplied unconjugated at 0.5 mg/mL, while the detection antibody is a sheep polyclonal antibody supplied biotinylated at 0.5 mg/mL. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair is validated for use in ELISA. No other assay formats are specified for this product.
Both the capture and detection antibodies were raised against recombinant human sCD14 protein. The capture antibody was purified by Protein A or G, and the detection antibody was purified by antigen affinity.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.