Human S100B (S100B) Antibody Pair (STJAP00668)
General Information
| Short Description | This S100B antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 13F5/10F4 |
| Isotype | IgG1/IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative./Liquid in PBS containing 0.01% thimerosal as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | S100B |
| Gene ID | 6285 |
| Uniprot ID | S100B_HUMAN |
| Immunogen | A synthesized peptide derived from S100B |
Additional Info
| Tissue Specificity | Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. |
| Function | Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain. Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation. Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. |
| Protein Name | Protein S100-BS-100 Protein Beta ChainS-100 Protein Subunit BetaS100 Calcium-Binding Protein B |
| Database Links | Reactome: R-HSA-1251985Reactome: R-HSA-445989Reactome: R-HSA-879415Reactome: R-HSA-933542 |
| Cellular Localisation | CytoplasmNucleusSecretedSecretion Into The Medium Is Promoted By Interaction With Isoform Clstn3beta Of Clstn3 |
| Alternative Antibody Pair Names | Protein S100-B antibody pairS-100 Protein Beta Chain antibody pairS-100 Protein Subunit Beta antibody pairS100 Calcium-Binding Protein B antibody pairS100B antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human S100B (S100B) Antibody Pair (STJAP00668)
This antibody pair detects human S100B protein (UniProt P04271). Reactivity has been confirmed for human samples only.
This antibody pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Both antibodies are mouse monoclonal IgGs supplied as affinity-purified liquids at 1 mg/mL. Clone 13F5 is an IgG1 and clone 10F4 is an IgG2a. Neither antibody carries a conjugate or tag.
A dilution of 1:1000 is recommended for ELISA applications. Researchers may wish to perform additional titration to optimize conditions for their specific experimental setup.
The capture antibody (clone 13F5) is formulated in PBS containing 0.09% sodium azide, while the detection antibody (clone 10F4) is formulated in PBS containing 0.01% thimerosal. Researchers should take note of these preservatives if downstream applications are sensitive to azide or thimerosal.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.