Human S100B (Protein S100-B) Antibody Pair (STJAP00148)
General Information
| Short Description | This S100B antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | S100B |
| Gene ID | 6285 |
| Uniprot ID | S100B_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human S100B protein.Detection Immunogen: Recombinant Human S100B protein. |
Additional Info
| Function | Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain. Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation. Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. |
| Protein Name | Protein S100-BS-100 Protein Beta ChainS-100 Protein Subunit BetaS100 Calcium-Binding Protein B |
| Database Links | Reactome: R-HSA-1251985Reactome: R-HSA-445989Reactome: R-HSA-879415Reactome: R-HSA-933542 |
| Cellular Localisation | CytoplasmNucleusSecretedSecretion Into The Medium Is Promoted By Interaction With Isoform Clstn3beta Of Clstn3 |
| Alternative Antibody Pair Names | Protein S100-B antibody pairS-100 Protein Beta Chain antibody pairS-100 Protein Subunit Beta antibody pairS100 Calcium-Binding Protein B antibody pairS100B antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human S100B (Protein S100-B) Antibody Pair (STJAP00148)
This antibody pair detects human S100B (Protein S100-B, UniProt P04271). Reactivity is confirmed for human samples only.
This antibody pair is validated for use in ELISA. No other assay formats are specified for this product.
Both antibodies are mouse monoclonal antibodies supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotinylated. Both are purified by Protein A or G and formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
Both the capture and detection antibodies were raised against recombinant human S100B protein. No specific immunogen region is defined in the product details.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.