Human S100A8 (Protein S100-A8) Antibody Pair (STJAP00489)
General Information
| Short Description | This S100A8 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Sheep/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | S100A8 |
| Gene ID | 6279 |
| Uniprot ID | S10A8_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human S100A8 protien.Detection Immunogen: Recombinant Human S100A8 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Also participates in regulatory T-cell differentiation together with CD69. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect.regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a IL-x-C-x-x-DE motif.S100A8 seems to contribute to S-nitrosylation site selectivity. (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner. |
| Protein Name | Protein S100-A8Calgranulin-ACalprotectin L1l SubunitCystic Fibrosis AntigenCfagLeukocyte L1 Complex Light ChainMigration Inhibitory Factor-Related Protein 8Mrp-8P8S100 Calcium-Binding Protein A8Urinary Stone Protein Band A |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-166058Reactome: R-HSA-5602498Reactome: R-HSA-5603041Reactome: R-HSA-5668599Reactome: R-HSA-5686938Reactome: R-HSA-6798695Reactome: R-HSA-6799990 |
| Cellular Localisation | SecretedCytoplasmCytoskeletonCell MembranePeripheral Membrane ProteinPredominantly Localized In The CytoplasmUpon Elevation Of The Intracellular Calcium LevelTranslocated From The Cytoplasm To The Cytoskeleton And The Cell MembraneUpon Neutrophil Activation Or Endothelial Adhesion Of MonocytesIs Secreted Via A Microtubule-MediatedAlternative Pathway |
| Alternative Antibody Pair Names | Protein S100-A8 antibody pairCalgranulin-A antibody pairCalprotectin L1l Subunit antibody pairCystic Fibrosis Antigen antibody pairCfag antibody pairLeukocyte L1 Complex Light Chain antibody pairMigration Inhibitory Factor-Related Protein 8 antibody pairMrp-8 antibody pairP8 antibody pairS100 Calcium-Binding Protein A8 antibody pairUrinary Stone Protein Band A antibody pairS100A8 antibody pairCAGA antibody pairCFAG antibody pairMRP8 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human S100A8 (Protein S100-A8) Antibody Pair (STJAP00489)
This antibody pair detects human S100A8 (Protein S100-A8, UniProt P05109) and has confirmed reactivity with human samples only. It is not validated for use with non-human species.
The capture antibody is an unconjugated sheep-raised antibody supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is a biotin-conjugated rabbit-raised antibody supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
This antibody pair has been validated for use in ELISA. No other assay formats are listed for this product.
Both the capture and detection antibodies were raised against recombinant human S100A8 protein. Both antibodies have been purified by antigen affinity chromatography.
This antibody pair is suitable for use with serum, plasma, and other biological fluids. It is intended for research use only and is not validated for diagnostic applications.
Both antibodies should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.