Human S100A4 (S100A4) Antibody Pair (STJAP00819)
General Information
| Short Description | This S100A4 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R54-1P-4/R62-2M-9 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | S100A4 |
| Gene ID | 6275 |
| Uniprot ID | S10A4_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Ubiquitously expressed. |
| Function | Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy. Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9. Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis. Also modulates the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels. Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes. In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors. |
| Protein Name | Protein S100-A4CalvasculinMetastasinPlacental Calcium-Binding ProteinProtein Mts1S100 Calcium-Binding Protein A4 |
| Database Links | |
| Cellular Localisation | SecretedNucleusCytoplasm |
| Alternative Antibody Pair Names | Protein S100-A4 antibody pairCalvasculin antibody pairMetastasin antibody pairPlacental Calcium-Binding Protein antibody pairProtein Mts1 antibody pairS100 Calcium-Binding Protein A4 antibody pairS100A4 antibody pairCAPL antibody pairMTS1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human S100A4 (S100A4) Antibody Pair (STJAP00819)
This antibody pair is designed to detect human S100A4 protein, corresponding to UniProt accession P26447. Reactivity has been confirmed for human samples only.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. The capture clone is R54-1P-4 and the detection clone is R62-2M-9.
This antibody pair is validated for use in ELISA. It is not listed for other assay formats such as lateral flow or multiplex immunoassay.
Optimal working concentrations are to be determined by the end user and are not predefined in the supplied product data. Titration experiments are recommended to establish appropriate conditions for each specific assay setup.
Both components should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C. Repeated freeze/thaw cycles should be avoided to maintain activity.
Both monoclonal antibodies were raised in rabbit using a recombinant human S100A4 protein as the immunogen. No additional information on the specific immunogen region is provided in the current product data.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.