Human S100A11 (Protein S100-A11) CLIA Kit for Quantitative Detection

SPECIFICATIONS
STJC0000594
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General Information

Short DescriptionThis human S100A11 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
ApplicationsCLIA
ReactivityHuman
Sensitivity37.5pg/mL
Detection Limit62.5~4000pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionChemiluminescence

Target Information

Gene SymbolS100A11
Gene ID6282
Uniprot IDS10AB_HUMAN
SpecificityThis kit recognizes Human S100A11 in samples. No significant cross-reactivity or interference between Human S100A11 and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Post Translational Modifications Phosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation.
Function Facilitates the differentiation and the cornification of keratinocytes.
Protein Name Protein S100-A11
Calgizzarin
Metastatic Lymph Node Gene 70 Protein
Mln 70
Protein S100-C
S100 Calcium-Binding Protein A11 Cleaved Into - Protein S100-A11 - N-Terminally Processed
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Nucleus
Alternative CLIA Names Protein S100-A11 CLIA kit
Calgizzarin CLIA kit
Metastatic Lymph Node Gene 70 Protein CLIA kit
Mln 70 CLIA kit
Protein S100-C CLIA kit
S100 Calcium-Binding Protein A11 Cleaved Into - Protein S100-A11 - N-Terminally Processed CLIA kit
S100A11 CLIA kit
MLN70 CLIA kit
S100C CLIA kit
Specificity This kit recognizes Human S100A11 in samples. No significant cross-reactivity or interference between Human S100A11 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-109 102
EDTA plasma(n=8) 91-106 99
Cell culture media(n=8) 93-107 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 218.13 537.35 1645.33 238.26 510.96 1712.52
Standard deviation 21.97 56.80 148.41 27.23 40.06 132.72
CV (%) 10.07 10.57 9.02 11.43 7.84 7.75

Citations

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