Human RUNX2 (Runt-related transcription factor 2) Sandwich ELISA Kit (STJE0002592)

Name: Human RUNX2 (Runt-related transcription factor 2) Sandwich ELISA Kit (STJE0002592)
SKU: STJE0002592
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0002592

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General Information

Short Description	This RUNX2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.19ng/mL
Detection Limit	0.31~20ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	RUNX2
Gene ID	860
Uniprot ID	RUNX2_HUMAN
Specificity	This kit recognizes Human RUNX2 in samples. No significant cross-reactivity or interference between Human RUNX2 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Post Translational Modifications	Phosphorylated.probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation. Phosphorylation at Ser-451 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. Isoform 3 is phosphorylated on Ser-340.
Function	Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation.
Protein Name	Runt-Related Transcription Factor 2 Acute Myeloid Leukemia 3 Protein Core-Binding Factor Subunit Alpha-1 Cbf-Alpha-1 Oncogene Aml-3 Osteoblast-Specific Transcription Factor 2 Osf-2 Polyomavirus Enhancer-Binding Protein 2 Alpha A Subunit Pea2-Alpha A Pebp2-Alpha A Sl3-3 Enhancer Factor 1 Alpha A Subunit Sl3/Akv Core-Binding Factor Alpha A Subunit
Database Links	Reactome: R-HSA-2032785 Reactome: R-HSA-8878166 Reactome: R-HSA-8939246 Reactome: R-HSA-8939902 Reactome: R-HSA-8940973 Reactome: R-HSA-8941284 Reactome: R-HSA-8941326 Reactome: R-HSA-8941332 Reactome: R-HSA-8941333
Cellular Localisation	Nucleus Cytoplasm
Alternative ELISA Names	Runt-Related Transcription Factor 2 ELISA kit Acute Myeloid Leukemia 3 Protein ELISA kit Core-Binding Factor Subunit Alpha-1 ELISA kit Cbf-Alpha-1 ELISA kit Oncogene Aml-3 ELISA kit Osteoblast-Specific Transcription Factor 2 ELISA kit Osf-2 ELISA kit Polyomavirus Enhancer-Binding Protein 2 Alpha A Subunit ELISA kit Pea2-Alpha A ELISA kit Pebp2-Alpha A ELISA kit Sl3-3 Enhancer Factor 1 Alpha A Subunit ELISA kit Sl3/Akv Core-Binding Factor Alpha A Subunit ELISA kit RUNX2 ELISA kit AML3 ELISA kit CBFA1 ELISA kit OSF2 ELISA kit PEBP2A ELISA kit
Specificity	This kit recognizes Human RUNX2 in samples. No significant cross-reactivity or interference between Human RUNX2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-102	96
EDTA plasma (n=8)	87-102	94
Cell culture media (n=8)	93-111	101

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.04	2.52	6.92	1.09	2.50	6.44
Standard deviation	0.06	0.14	0.32	0.07	0.12	0.26
CV (%)	5.77	5.56	4.62	6.42	4.80	4.04

Citations

View product citations for antibody STJE0002592 on CiteAb