Human RPS3 protein (Recombinant) (N-His) (STJP007544)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human RPS3-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | RPS3 |
| Gene ID | 6188 |
| Uniprot ID | RS3_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Lys187 |
Additional Info
| Function | Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Has endonuclease activity and plays a role in repair of damaged DNA. Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA. Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS). Has also been shown to bind with similar affinity to intact and damaged DNA. Stimulates the N-glycosylase activity of the base excision protein OGG1. Enhances the uracil excision activity of UNG1. Also stimulates the cleavage of the phosphodiester backbone by APEX1. When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage. Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide. Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes. Represses its own translation by binding to its cognate mRNA. Binds to and protects TP53/p53 from MDM2-mediated ubiquitination. Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization. Involved in induction of apoptosis through its role in activation of CASP8. Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5. Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation. |
| Protein Name | Small Ribosomal Subunit Protein Us340s Ribosomal Protein S3 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-156902Reactome: R-HSA-1799339Reactome: R-HSA-192823Reactome: R-HSA-2408557Reactome: R-HSA-6791226Reactome: R-HSA-72649Reactome: R-HSA-72689Reactome: R-HSA-72695Reactome: R-HSA-72702Reactome: R-HSA-72706Reactome: R-HSA-72764Reactome: R-HSA-9010553Reactome: R-HSA-9633012Reactome: R-HSA-9735869Reactome: R-HSA-9754678Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | CytoplasmNucleusNucleolusMitochondrion Inner MembranePeripheral Membrane ProteinCytoskeletonSpindleIn Normal CellsLocated Mainly In The Cytoplasm With Small Amounts In The Nucleus But Translocates To The Nucleus In Cells Undergoing ApoptosisNuclear Translocation Is Induced By Dna Damaging Agents Such As Hydrogen PeroxideAccumulates In The Mitochondrion In Response To Increased Ros LevelsLocalizes To The Spindle During MitosisLocalized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas |
| Alternative Protein Names | Small Ribosomal Subunit Protein Us3 protein40s Ribosomal Protein S3 proteinRPS3 proteinOK proteinSW-cl.26 protein |
Information sourced from Uniprot.org