Human RNF20 protein (Recombinant) (N-His-SUMO & C-Strep) (STJP008128)

SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
STJP008128
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General Information

Short DescriptionRecombinant-Human RNF20-N-His-SUMO & C-Strep protein was developed from e.coli for the region N-His-SUMO & C-Strep. For use in research applications.
ApplicationsELISA/Immunogen/SDS-PAGE/WB
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Dilution RangeReconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
FormulationLyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Storage InstructionUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt.

Target Information

Gene SymbolRNF20
Gene ID56254
Uniprot IDBRE1A_HUMAN
ImmunogenHomo sapiens (Human)
Immunogen RegionAsp906-Gly975

Additional Info

Function Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B.reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. (Microbial infection) Promotes the human herpesvirus 8 (KSHV) lytic cycle by inducing the expression of lytic viral genes including the latency switch gene RTA/ORF50.
Protein Name E3 Ubiquitin-Protein Ligase Bre1a
Bre1-A
Hbre1
Ring Finger Protein 20
Ring-Type E3 Ubiquitin Transferase Bre1a
Database Links Reactome: R-HSA-8866654
Reactome: R-HSA-9013422
Cellular Localisation Nucleus
Alternative Protein Names E3 Ubiquitin-Protein Ligase Bre1a protein
Bre1-A protein
Hbre1 protein
Ring Finger Protein 20 protein
Ring-Type E3 Ubiquitin Transferase Bre1a protein
RNF20 protein
BRE1A protein

Information sourced from Uniprot.org

Citations

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