Human RNF114 protein (Recombinant) (His-Tag) (STJP018700)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human RNF114-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Denatured |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF114 |
| Gene ID | 55905 |
| Uniprot ID | RN114_HUMAN |
| Accession Number | NP_061153 |
| Immunogen | Human |
| Immunogen Region | 1-228aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Expressed in numerous tissues, including skin, CD4 lymphocytes and dendritic cells. Highest levels in testis. |
| Post Translational Modifications | Autoubiquitinated. Polyubiquitinated in the presence of E2 enzymes UBE2D1, UBE2D2 and UBE2D3, but only monoubiquitinated in the presence of UBE2E1. |
| Function | E3 ubiquitin-protein ligase that promotes the ubiquitination of various substrates. In turn, participates in the regulation of many biological processes including cell cycle, apoptosis, osteoclastogenesis as well as innate or adaptive immunity. Acts as a negative regulator of NF-kappa-B-dependent transcription by promoting the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3. May promote the ubiquitination of TRAF6 as well. Also acts as a negative regulator of T-cell activation. Inhibits cellular dsRNA responses and interferon production by targeting MAVS component for proteasomal degradation. Ubiquitinates the CDK inhibitor CDKN1A leading to its degradationand probably also CDKN1B and CDKN1C. This activity stimulates cell cycle G1-to-S phase transition and suppresses cellular senescence. May play a role in spermatogenesis. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf114Ring Finger Protein 114Ring-Type E3 Ubiquitin Transferase Rnf114Zinc Finger Protein 228Zinc Finger Protein 313 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Protein Names | E3 Ubiquitin-Protein Ligase Rnf114 proteinRing Finger Protein 114 proteinRing-Type E3 Ubiquitin Transferase Rnf114 proteinZinc Finger Protein 228 proteinZinc Finger Protein 313 proteinRNF114 proteinZNF228 proteinZNF313 protein |
Information sourced from Uniprot.org