Human RLN2 (Prorelaxin H2) Sandwich ELISA Kit (STJE0003079)

SPECIFICATIONS
STJE0003079
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General Information

Short DescriptionThis RLN2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityHuman
Sensitivity4.69pg/mL
Detection Limit7.81~500pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolRLN2
Gene ID6019
Uniprot IDREL2_HUMAN
SpecificityThis kit recognizes Human RLN2 in samples. No significant cross-reactivity or interference between Human RLN2 and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Function Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.
Protein Name Prorelaxin H2 Cleaved Into - Relaxin B Chain - Relaxin A Chain
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-444821
Cellular Localisation Secreted
Alternative ELISA Names Prorelaxin H2 Cleaved Into - Relaxin B Chain - Relaxin A Chain ELISA kit
RLN2 ELISA kit
Specificity This kit recognizes Human RLN2 in samples. No significant cross-reactivity or interference between Human RLN2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 94
EDTA plasma (n=8) 91-105 96
Cell culture media (n=8) 89-106 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 25.40 43.71 244.95 27.29 41.78 265.59
Standard deviation 1.61 2.57 9.46 1.54 2.34 14.24
CV (%) 6.34 5.88 3.86 5.64 5.60 5.36

Citations

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