Human RET protein (Recombinant) (N-His) (STJP003811)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human RET-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | RET |
| Gene ID | 5979 |
| Uniprot ID | RET_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Leu29-Arg635 |
Additional Info
| Function | Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation in response to glia cell line-derived growth family factors (GDNF, NRTN, ARTN, PSPN and GDF15). In contrast to most receptor tyrosine kinases, RET requires not only its cognate ligands but also coreceptors, for activation. GDNF ligands (GDNF, NRTN, ARTN, PSPN and GDF15) first bind their corresponding GDNFR coreceptors (GFRA1, GFRA2, GFRA3, GFRA4 and GFRAL, respectively), triggering RET autophosphorylation and activation, leading to activation of downstream signaling pathways, including the MAPK- and AKT-signaling pathways. Acts as a dependence receptor via the GDNF-GFRA1 signaling: in the presence of the ligand GDNF in somatotrophs within pituitary, promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Required for the molecular mechanisms orchestration during intestine organogenesis via the ARTN-GFRA3 signaling: involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Also active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Involved in the development of the neural crest. Regulates nociceptor survival and size. Phosphorylates PTK2/FAK1. Isoform 1: Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL. |
| Protein Name | Proto-Oncogene Tyrosine-Protein Kinase Receptor RetCadherin Family Member 12Proto-Oncogene C-Ret Cleaved Into - Soluble Ret Kinase Fragment - Extracellular Cell-Membrane Anchored Ret Cadherin 120 Kda Fragment |
| Database Links | Reactome: R-HSA-5673001Reactome: R-HSA-8853659Reactome: R-HSA-9768919Reactome: R-HSA-9830364Reactome: R-HSA-9830674 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndosome MembranePredominantly Located On The Plasma MembraneIn The Presence Of Sorl1 And Gfra1Directed To Endosomes |
| Alternative Protein Names | Proto-Oncogene Tyrosine-Protein Kinase Receptor Ret proteinCadherin Family Member 12 proteinProto-Oncogene C-Ret Cleaved Into - Soluble Ret Kinase Fragment - Extracellular Cell-Membrane Anchored Ret Cadherin 120 Kda Fragment proteinRET proteinCDHF12 proteinCDHR16 proteinPTC protein |
Information sourced from Uniprot.org