| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Recombinant-Human Renin-protein was developed from hek293. For use in research applications. |
| Conjugation: | Unconjugated |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The activity was tested by the ability to digest a fluorogenic substrate and measuered to be 22 pmoles/min/ug NA |
| Gene Symbol: | DKK1 |
| Gene ID: | 22943 |
| Uniprot ID: | DKK1_HUMAN |
| Immunogen Region: | LEU24-ARG406, ECD |
| Immunogen: | Optimized DNA sequence encoding extracellular domain of Human Renin (LEU24-ARG406) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Placenta. |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity. |
| Protein Name | Dickkopf-Related Protein 1 Dickkopf-1 Dkk-1 Hdkk-1 Sk |
| Database Links | Reactome: R-HSA-201681 Reactome: R-HSA-3772470 Reactome: R-HSA-5339717 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Dickkopf-Related Protein 1 protein Dickkopf-1 protein Dkk-1 protein Hdkk-1 protein Sk protein DKK1 protein UNQ492 protein PRO1008 protein |
Information sourced from Uniprot.org
