This RBM20 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids..
Applications
ELISA
Reactivity
Human
Sensitivity
0.121ng/mL
Detection Limit
0.312-20ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
tissue homogenates, cell lysates or other biological fluids.
Additional Info
Tissue Specificity
Mainly expressed in the heart. Also expressed in skeletal muscle tissues, ovary, small intestine and colon.
Post Translational Modifications
Phosphorylation regulates the subcellular localization. Phosphorylation of Ser-635 and Ser-637 in the RS (arginine/serine-rich) region promotes nuclear localization of the protein. In contrast, phosphorylation of the C-terminal disordered region promotes localization to cytoplasmic ribonucleoprotein granules.
Function
RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes encoding key structural proteins involved in cardiac development, such as TTN (Titin), CACNA1C, CAMK2D or PDLIM5/ENH. Acts as a repressor of mRNA splicing: specifically binds the 5'UCUU-3' motif that is predominantly found within intronic sequences of pre-mRNAs, leading to the exclusion of specific exons in target transcripts. RBM20-mediated exon skipping is hormone-dependent and is essential for TTN isoform transition in both cardiac and skeletal muscles. RBM20-mediated exon skipping of TTN provides substrates for the formation of circular RNA (circRNAs) from the TTN transcripts. Together with RBM24, promotes the expression of short isoforms of PDLIM5/ENH in cardiomyocytes.
Protein Name
Rna-Binding Protein 20Rna-Binding Motif Protein 20
Database Links
Cellular Localisation
NucleusCytoplasmCytoplasmic Ribonucleoprotein GranuleThe Active Form That Regulates Alternative Splicing Localizes To The NucleusAlso Localizes To Cytoplasmic Ribonucleoprotein GranulesLocalization To Cytoplasmic Ribonucleoprotein Granules Plays An Important Regulatory RoleSubcellular Localization Is Regulated By Phosphorylation Of Different Parts Of The Protein: While Phosphorylation Of The Rs (Arginine/Serine-Rich) Region Promotes Nuclear LocalizationPhosphorylation Of The C-Terminal Disordered Region Promotes Localization To Cytoplasmic Ribonucleoprotein Granules
Alternative ELISA Names
Rna-Binding Protein 20 ELISA kitRna-Binding Motif Protein 20 ELISA kitRBM20 ELISA kit
