Human RANTES (RANTES) Antibody Pair (STJAP00735)
General Information
| Short Description | This RANTES antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R06-8V9/R03-8P5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CCL5 |
| Gene ID | 6352 |
| Uniprot ID | CCL5_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in the follicular fluid (at protein level). T-cell and macrophage specific. |
| Post Translational Modifications | N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells. N-terminal processed form RANTES(4-68) is produced by proteolytic cleavage by cathepsin CTSG. The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity. |
| Function | Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells. |
| Protein Name | C-C Motif Chemokine 5EocpEosinophil Chemotactic CytokineSis-DeltaSmall-Inducible Cytokine A5T Cell-Specific Protein P228Tcp228T-Cell-Specific Protein Rantes Cleaved Into - Rantes(3-68 - Rantes(4-68 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-6783783 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | C-C Motif Chemokine 5 antibody pairEocp antibody pairEosinophil Chemotactic Cytokine antibody pairSis-Delta antibody pairSmall-Inducible Cytokine A5 antibody pairT Cell-Specific Protein P228 antibody pairTcp228 antibody pairT-Cell-Specific Protein Rantes Cleaved Into - Rantes(3-68 - Rantes(4-68 antibody pairCCL5 antibody pairD17S136E antibody pairSCYA5 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human RANTES (RANTES) Antibody Pair (STJAP00735)
This antibody pair is designed to detect human RANTES (CCL5), corresponding to UniProt entry P13501. Reactivity is confirmed for human samples only.
This antibody pair is validated for use in ELISA. It is intended for sandwich ELISA development where one antibody serves as the capture and the other as the detection reagent.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and affinity purified. Clone IDs are R06-8V9 and R03-8P5 respectively.
Optimal working concentrations are determined by the end user and are not predefined in the product specifications. Titration experiments are recommended to establish the appropriate dilution for each antibody within the specific assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
This product is for research use only and is not approved or validated for diagnostic, therapeutic, or clinical applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.