Human RANTES (C-C Motif Chemokine 5) Antibody Pair (STJAP00191)
General Information
| Short Description | This RANTES antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | CCL5 |
| Gene ID | 6352 |
| Uniprot ID | CCL5_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human RANTES protein.Detection Immunogen: Recombinant Human RANTES protein. |
Additional Info
| Post Translational Modifications | N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells. N-terminal processed form RANTES(4-68) is produced by proteolytic cleavage by cathepsin CTSG. The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity. |
| Function | Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells. |
| Protein Name | C-C Motif Chemokine 5EocpEosinophil Chemotactic CytokineSis-DeltaSmall-Inducible Cytokine A5T Cell-Specific Protein P228Tcp228T-Cell-Specific Protein Rantes Cleaved Into - Rantes(3-68 - Rantes(4-68 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-6783783 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | C-C Motif Chemokine 5 antibody pairEocp antibody pairEosinophil Chemotactic Cytokine antibody pairSis-Delta antibody pairSmall-Inducible Cytokine A5 antibody pairT Cell-Specific Protein P228 antibody pairTcp228 antibody pairT-Cell-Specific Protein Rantes Cleaved Into - Rantes(3-68 - Rantes(4-68 antibody pairCCL5 antibody pairD17S136E antibody pairSCYA5 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human RANTES (C-C Motif Chemokine 5) Antibody Pair (STJAP00191)
This antibody pair detects human RANTES (C-C Motif Chemokine 5, UniProt P13501) and is validated for human reactivity only. Both the capture and detection antibodies were raised against recombinant human RANTES protein.
The capture antibody is a mouse monoclonal unconjugated antibody, while the detection antibody is a goat polyclonal antibody conjugated to biotin. Both components are supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair is validated for use in ELISA only. It is intended for research use and is not validated for diagnostic or therapeutic applications.
The capture antibody was purified by Protein A or G affinity chromatography, while the detection antibody was purified by antigen affinity chromatography. Both antibodies were raised against recombinant human RANTES protein as the immunogen.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity and performance.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.