Human RAD51AP1 protein (Recombinant) (N-His-SUMO) (STJP008075)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human RAD51AP1-N-His-SUMO protein was developed from e.coli for the region N-His-SUMO. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | RAD51AP1 |
| Gene ID | 10635 |
| Uniprot ID | R51A1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Arg70-Asn116 |
Additional Info
| Post Translational Modifications | Sumoylation with SUMO2/3 by NSMCE2/MMS21 promotes stabilization, possibly by preventing ubiquitination. Sumoylation is required for alternative lengthening of telomeres (ALT) pathway. |
| Function | Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination. Acts by stimulating D-Loop formation by RAD51: specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51. Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures: has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation. Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair. WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2. Also involved in meiosis by promoting DMC1-mediated homologous meiotic recombination. Key mediator of alternative lengthening of telomeres (ALT) pathway, a homology-directed repair mechanism of telomere elongation that controls proliferation in aggressive cancers, by stimulating homologous recombination. May also bind RNA.additional evidences are however required to confirm RNA-binding in vivo. |
| Protein Name | Rad51-Associated Protein 1Hsrad51ap1Rad51-Interacting Protein |
| Database Links | Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709603 |
| Cellular Localisation | ChromosomeNucleusTelomereColocalizes With Rad51 To Multiple Nuclear FociColocalizes With Dmc1 On Meiotic Chromatin |
| Alternative Protein Names | Rad51-Associated Protein 1 proteinHsrad51ap1 proteinRad51-Interacting Protein proteinRAD51AP1 proteinPIR51 protein |
Information sourced from Uniprot.org