This RAB7A Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates or other biological fluids..
Applications
ELISA
Reactivity
Human
Sensitivity
0.113ng/mL
Detection Limit
0.312-20ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
serum, plasma, tissue homogenates or other biological fluids.
Additional Info
Tissue Specificity
Widely expressed.high expression found in skeletal muscle.
Post Translational Modifications
Deubiquitination at Lys-191 and Lys-194 by USP32. Phosphorylated at Ser-72 by LRRK1.phosphorylation is dependent on protein kinase C (PKC) activation of LRRK1. Prenylated. Prenylation is required for association with cellular membranes.
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. In its active state, RAB7A binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Also plays a central role in growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway. Required for the exosomal release of SDCBP, CD63 and syndecan. Required for vesicular trafficking and cell surface expression of ACE2. May play a role in PRPH neuronal intermediate filament assembly.
Cytoplasmic VesiclePhagosome MembranePeripheral Membrane ProteinCytoplasmic SideLate Endosome MembraneLysosome MembraneMelanosome MembraneAutophagosome MembraneLipid DropletEndosome MembraneMitochondrion MembraneColocalizes With Osbpl1a At The Late EndosomeFound In The Ruffled Border (A Late Endosomal-Like Compartment In The Plasma Membrane) Of Bone-Resorbing OsteoclastsRecruited To Phagosomes Containing SAureus Or MycobacteriumLipid Droplet Localization Is Increased Upon Adrb2 StimulationRecruited To Damaged Mitochondria During Mitophagy In A Rimoc1-Dependent Manner
Alternative ELISA Names
Ras-Related Protein Rab-7a ELISA kitRAB7A ELISA kitRAB7 ELISA kit
