Human RAB7A protein (Recombinant) (N-His) (STJP004860)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human RAB7A-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | RAB7A |
| Gene ID | 7879 |
| Uniprot ID | RAB7A_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Cys207 |
Additional Info
| Post Translational Modifications | Deubiquitination at Lys-191 and Lys-194 by USP32. Phosphorylated at Ser-72 by LRRK1.phosphorylation is dependent on protein kinase C (PKC) activation of LRRK1. Prenylated. Prenylation is required for association with cellular membranes. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. In its active state, RAB7A binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Also plays a central role in growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway. Required for the exosomal release of SDCBP, CD63 and syndecan. Required for vesicular trafficking and cell surface expression of ACE2. May play a role in PRPH neuronal intermediate filament assembly. |
| Protein Name | Ras-Related Protein Rab-7a |
| Database Links | Reactome: R-HSA-2132295Reactome: R-HSA-6798695Reactome: R-HSA-8854214Reactome: R-HSA-8873719Reactome: R-HSA-8876198Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013406Reactome: R-HSA-9013407Reactome: R-HSA-9013408Reactome: R-HSA-9013409Reactome: R-HSA-9013423Reactome: R-HSA-9035034Reactome: R-HSA-9636383Reactome: R-HSA-9636569 |
| Cellular Localisation | Cytoplasmic VesiclePhagosome MembranePeripheral Membrane ProteinCytoplasmic SideLate Endosome MembraneLysosome MembraneMelanosome MembraneAutophagosome MembraneLipid DropletEndosome MembraneMitochondrion MembraneColocalizes With Osbpl1a At The Late EndosomeFound In The Ruffled Border (A Late Endosomal-Like Compartment In The Plasma Membrane) Of Bone-Resorbing OsteoclastsRecruited To Phagosomes Containing SAureus Or MycobacteriumLipid Droplet Localization Is Increased Upon Adrb2 StimulationRecruited To Damaged Mitochondria During Mitophagy In A Rimoc1-Dependent Manner |
| Alternative Protein Names | Ras-Related Protein Rab-7a proteinRAB7A proteinRAB7 protein |
Information sourced from Uniprot.org