Human RAB21 protein (Recombinant) (His-Tag) (STJP017045)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP017045
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General Information

Short DescriptionRecombinant-Human RAB21-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.5 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 2mM DTT, 200mM NaCl, 1mM PMSF
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolRAB21
Gene ID23011
Uniprot IDRAB21_HUMAN
Accession NumberNP_055814.1
ImmunogenHuman
Immunogen Region18-222aa
Immunogen SequenceYSF KVVLLGEGCV GKTSLVLRYC ENKFNDKHIT TLQASFLTKK LNIGGKRVNL AIWDTAGQER FHALGPIYYR DSNGAILVYD ITDEDSFQKV KNWVKELRKM LGNEICLCIV GNKIDLEKER HVSIQEAESY AESVGAKHYH TSAKQNKGIE ELFLDLCKRM IETAQVDERA KGNGSSQPGT ARRGVQ

Additional Info

Tissue Specificity Widely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB21 is involved in membrane trafficking control. During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration. Involved in neurite growth. Following SBF2/MTMT13-mediated activation in response to starvation-induced autophagy, binds to and regulates SNARE protein VAMP8 endolysosomal transport required for SNARE-mediated autophagosome-lysosome fusion. Modulates protein levels of the cargo receptors TMED2 and TMED10, and required for appropriate Golgi localization of TMED10.
Protein Name Ras-Related Protein Rab-21
Database Links Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Endoplasmic Reticulum Membrane
Lipid-Anchor
Golgi Apparatus
Trans-Golgi Network
Golgi Apparatus Membrane
Early Endosome Membrane
Cytoplasmic Vesicle Membrane
Cleavage Furrow
Cell Projection
Neuron Projection
Colocalizes With Ankrd27 And Vamp7 In Neurites
In Nonpolarized Epithelial Caco-2 Cells
Found In The Endoplasmic Reticulum
In Polarized Cells
Observed In Vesicles In The Apical Cytoplasm
During Mitosis
In Mid-Telophase
Localized In The Ingressing Cleavage Furrow
In Late Telophase
Detected At The Opposite Poles Of The Daughter Cells
In Vesicles At The Base Of Lamellipodia Formed By The Separating Daughter Cells
Alternative Protein Names Ras-Related Protein Rab-21 protein
RAB21 protein
KIAA0118 protein

Information sourced from Uniprot.org

Citations

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