Human PVR/CD155 protein (Recombinant) (C-hFc&His) (STJP001080)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human PVR/CD155 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Asp28-Asn343) of human CD155/PVR (Accession #NP_006496.4) fused with an Fc, 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human PVR/CD155-C-hFc&His protein was developed in hek293 cells using the region Asp28-Asn343. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human CD155 at 2 Mu g/mL (100 Mu L/well) can bind Human CD96 with a linear range of 0.1-76 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | PVR |
| Gene ID | 5817 |
| Uniprot ID | PVR_HUMAN |
| Immunogen | Recombinant Human PVR/CD155 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Asp28-Asn343) of human CD155/PVR (Accession #NP_006496.4) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Region | Asp28-Asn343 |
| Immunogen Sequence | DVVVQAPTQVPGFLGDSVTL PCYLQVPNMEVTHVSQLTWA RHGESGSMAVFHQTQGPSYS ESKRLEFVAARLGAELRNAS LRMFGLRVEDEGNYTCLFVT FPQGSRSVDIWLRVLAKPQN TAEVQKVQLTGEPVPMARCV STGGRPPAQITWHSDLGGMP NTSQVPGFLSGTVTVTSLWI LVPSSQVDGKNVTCKVEHES FEKPQLLTVNLTVYYPPEVS ISGYDNNWYLGQNEATLTC |
Additional Info
| Post Translational Modifications | N-glycosylated. N-glycan at Asn-120: Hex5HexNAc4. Phosphorylated by Src kinases on tyrosine residues in the ITIM motif upon ligation. Interaction with TIGIT is required for Phosphorylation. |
| Function | Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. (Microbial infection) Acts as a receptor for Pseudorabies virus. (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells. |
| Protein Name | Poliovirus ReceptorNectin-Like Protein 5Necl-5Cd Antigen Cd155 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-420597 |
| Cellular Localisation | Isoform Alpha: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Delta: Cell MembraneIsoform Beta: SecretedIsoform Gamma: Secreted |
| Alternative Protein Names | Poliovirus Receptor proteinNectin-Like Protein 5 proteinNecl-5 proteinCd Antigen Cd155 proteinPVR proteinPVS protein |
Information sourced from Uniprot.org