Human PTTG1 protein (Recombinant) (His-Tag) (STJP016508)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human PTTG1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% Glycerol, 1mM EDTA, 0.1mM PMSF |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
Target Information
| Gene Symbol | PTTG1 |
| Gene ID | 9232 |
| Uniprot ID | PTTG1_HUMAN |
| Accession Number | NP_004210 |
| Immunogen | Human |
| Immunogen Region | 1-202aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI |
Additional Info
| Tissue Specificity | Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer. |
| Post Translational Modifications | Phosphorylated at Ser-165 by CDK1 during mitosis. Phosphorylated in vitro by ds-DNA kinase. Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10. |
| Function | Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. |
| Protein Name | SecurinEsp1-Associated ProteinPituitary Tumor-Transforming Gene 1 ProteinTumor-Transforming Protein 1Hpttg |
| Database Links | Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-2467813 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Protein Names | Securin proteinEsp1-Associated Protein proteinPituitary Tumor-Transforming Gene 1 Protein proteinTumor-Transforming Protein 1 proteinHpttg proteinPTTG1 proteinEAP1 proteinPTTG proteinTUTR1 protein |
Information sourced from Uniprot.org