Human PSME3 (Proteasome Activator Subunit 3) Sandwich ELISA Kit (STJE0011717)

SPECIFICATIONS
STJE0011717
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General Information

Short DescriptionThis PSME3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids..
ApplicationsELISA
ReactivityHuman
Sensitivity0.054ng/mL
Detection Limit0.156-10ng/mL
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Storage InstructionStore the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time4.5 hrs

Target Information

Gene SymbolPSME3
Gene ID10197
Uniprot IDPSME3_HUMAN
Sample Typetissue homogenates, cell lysates or other biological fluids.

Additional Info

Tissue Specificity
Post Translational Modifications Phosphorylated by MAP3K3. Phosphorylation at Ser-247 promotes its association with CCAR2. Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1.
Function Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition.
Protein Name Proteasome Activator Complex Subunit 3
11s Regulator Complex Subunit Gamma
Reg-Gamma
Activator Of Multicatalytic Protease Subunit 3
Ki Nuclear Autoantigen
Proteasome Activator 28 Subunit Gamma
Pa28g
Pa28gamma
Database Links Reactome: R-HSA-9907900
Cellular Localisation Nucleus
Cytoplasm
Localizes To The Cytoplasm During Mitosis Following Nuclear Envelope Breakdown At This Distinct Stage Of The Cell Cycle Which Allows Its Interaction With Map3k3 Kinase
Alternative ELISA Names Proteasome Activator Complex Subunit 3 ELISA kit
11s Regulator Complex Subunit Gamma ELISA kit
Reg-Gamma ELISA kit
Activator Of Multicatalytic Protease Subunit 3 ELISA kit
Ki Nuclear Autoantigen ELISA kit
Proteasome Activator 28 Subunit Gamma ELISA kit
Pa28g ELISA kit
Pa28gamma ELISA kit
PSME3 ELISA kit
output

Information sourced from Uniprot.org

Citations

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