Human PSMD10/Gankyrin protein (Recombinant) (Tag free) (STJP014292)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human PSMD10/Gankyrin-Tag free protein was developed from e.coli and has a target region of Tag free. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Supplied as a 0.22 Mu m filtered solution in PBS, 10% Glycerol, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
Target Information
| Gene Symbol | PSMD10 |
| Gene ID | 5716 |
| Uniprot ID | PSD10_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Gly226 |
Additional Info
| Function | Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis. Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export. |
| Protein Name | 26s Proteasome Non-Atpase Regulatory Subunit 1026s Proteasome Regulatory Subunit P28GankyrinP28(Gank |
| Database Links | Reactome: R-HSA-9907900 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Protein Names | 26s Proteasome Non-Atpase Regulatory Subunit 10 protein26s Proteasome Regulatory Subunit P28 proteinGankyrin proteinP28(Gank proteinPSMD10 protein |
Information sourced from Uniprot.org