Human Prostatic Acid Phosphatase/ACPP protein (Recombinant) (C-6His) (STJP020091)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Prostatic Acid Phosphatase/ACPP Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys33-Asp386) of Human Prostatic Acid Phosphatase/ACPP (Accession #NP_001090.2) fused with His at
General Information
| Short Description | Recombinant-Human Prostatic Acid Phosphatase/ACPP-C-6His protein was developed in hek293 cells using the region Lys33-Asp386. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | 0.22 Mu m filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5. |
| Storage Instruction | Store at-70 °C. This product is stable at ≤-70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
| Determination Method | < 0.01 EU/Mu g of the protein by LAL method |
Target Information
| Gene Symbol | ACP3 |
| Gene ID | 55 |
| Uniprot ID | PPAP_HUMAN |
| Immunogen | Recombinant Human Prostatic Acid Phosphatase/ACPP Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys33-Asp386) of Human Prostatic Acid Phosphatase/ACPP (Accession #NP_001090.2) fused with His at |
| Immunogen Region | Lys33-Asp386 |
| Immunogen Sequence | KELKFVTLVFRHGDRSPIDT FPTDPIKESSWPQGFGQLTQ LGMEQHYELGEYIRKRYRKF LNESYKHEQVYIRSTDVDRT LMSAMTNLAALFPPEGVSIW NPILLWQPIPVHTVPLSEDQ LLYLPFRNCPRFQELESETL KSEEFQKRLHPYKDFIATLG KLSGLHGQDLFGIWSKVYDP LYCESVHNFTLPSWATEDTM TKLRELSELSLLSLYGIHKQ KEKSRLQGGVLVNEILNHM |
Additional Info
| Tissue Specificity | Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Low expression in prostate carcinoma cells and tissues. Isoform 2: Widely expressed. Expressed in the sarcolemma of skeletal muscle. |
| Post Translational Modifications | N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection). |
| Function | A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Isoform 2: Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. PAPf39: (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection. |
| Protein Name | Prostatic Acid PhosphatasePap5'-Nucleotidase5'-NtAcid Phosphatase 3Ecto-5'-NucleotidaseProtein Tyrosine Phosphatase Acp3Thiamine MonophosphataseTmpase Cleaved Into - Papf39 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: Cell MembraneSingle-Pass Type I Membrane ProteinLysosome MembraneNucleusCytoplasmCytosolAppears To Shuttle Between The Cell Membrane And Intracellular VesiclesColocalizes With Flot1 At Cell Membrane And In Intracellular VesiclesColocalizes With Lamp2 On The Lysosome Membrane |
| Alternative Protein Names | Prostatic Acid Phosphatase proteinPap protein5'-Nucleotidase protein5'-Nt proteinAcid Phosphatase 3 proteinEcto-5'-Nucleotidase proteinProtein Tyrosine Phosphatase Acp3 proteinThiamine Monophosphatase proteinTmpase Cleaved Into - Papf39 proteinACP3 proteinACPP protein |
Information sourced from Uniprot.org