Human Progranulin (Progranulin) Antibody Pair (STJAP00910)
General Information
| Short Description | This Progranulin antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R09-3I-2/R03-5B-4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | GRN |
| Gene ID | 2896 |
| Uniprot ID | GRN_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney. |
| Post Translational Modifications | Cleaved by ELANE.proteolysis is blocked by SLPI and is concentration- and time-dependent and induces CXCL8/IL-8 production.granulin-3 and granulin-4 are resistant to ELANE. Cleaved by CTSL in lysosome thus regulating the maturation and turnover of progranulin within the lysosome. |
| Function | Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation. Regulates protein trafficking to lysosomes, and also the activity of lysosomal enzymes. Also facilitates the acidification of lysosomes, causing degradation of mature CTSD by CTSB. In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures. Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases. Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity. Granulin-4: Promotes proliferation of the epithelial cell line A431 in culture. Granulin-3: Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8. Granulin-7: Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity. |
| Protein Name | ProgranulinPgrnAcrograninEpithelin PrecursorGlycoprotein Of 88 KdaGp88Glycoprotein 88Granulin PrecursorPc Cell-Derived Growth FactorPcdgfProepithelinPepi Cleaved Into - Paragranulin - Granulin-1Granulin G - Granulin-2Granulin F - Granulin-3Epithelin-2Granulin B - Granulin-4Epithelin-1Granulin A - Granulin-5Granulin C - Granulin-6Granulin D - Granulin-7Granulin E |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | SecretedLysosomeEndocytosed By Sort1 And Delivred To LysosomesTargeted To Lysosome By Psap Via M6pr And Lrp1In Both Biosynthetic And Endocytic PathwaysCo-Localized With Gba1 In The Intracellular Trafficking Compartments Until To Lysosome |
| Alternative Antibody Pair Names | Progranulin antibody pairPgrn antibody pairAcrogranin antibody pairEpithelin Precursor antibody pairGlycoprotein Of 88 Kda antibody pairGp88 antibody pairGlycoprotein 88 antibody pairGranulin Precursor antibody pairPc Cell-Derived Growth Factor antibody pairPcdgf antibody pairProepithelin antibody pairPepi Cleaved Into - Paragranulin - Granulin-1 antibody pairGranulin G - Granulin-2 antibody pairGranulin F - Granulin-3 antibody pairEpithelin-2 antibody pairGranulin B - Granulin-4 antibody pairEpithelin-1 antibody pairGranulin A - Granulin-5 antibody pairGranulin C - Granulin-6 antibody pairGranulin D - Granulin-7 antibody pairGranulin E antibody pairGRN antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Progranulin (Progranulin) Antibody Pair (STJAP00910)
This antibody pair is designed to detect human Progranulin (UniProt P28799) and has confirmed reactivity with human samples only. No cross-reactivity data for other species is provided.
This antibody pair has been validated for use in ELISA. No other assay formats are listed as supported applications.
Both antibodies are rabbit monoclonal IgGs, with clone IDs R09-3I-2 and R03-5B-4, and both are supplied unconjugated. They are affinity-purified and formulated in 0.01M PBS at pH 7.2.
Working concentrations are determined by the end user, as no predefined dilution range is specified for this pair. Titration experiments are recommended to establish optimal concentrations for your specific assay conditions.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to preserve activity.
Both antibodies were raised against a recombinant protein immunogen corresponding to human Progranulin. No specific immunogen region is defined in the product details.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.