Human PRKDC protein (Recombinant) (N-His-GST) (STJP014581)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human PRKDC-N-His-GST protein was developed from e.coli and has a target region of N-His-GST. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for this information. |
Target Information
| Gene Symbol | PRKDC |
| Gene ID | 5591 |
| Uniprot ID | PRKDC_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ala3899-Met4128 |
Additional Info
| Function | Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). Recruited by XRCC5 and XRCC6 to DNA ends and is required to (1) protect and align broken ends of DNA, thereby preventing their degradation, (2) and sequester the DSB for repair by NHEJ. Acts as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome. Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome. Recognizes the substrate consensus sequence ST-Q. Phosphorylates 'Ser-139' of histone variant H2AX, thereby regulating DNA damage response mechanism. Phosphorylates ASF1A, DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, FH, SRF, NHEJ1/XLF, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Acts as a regulator of the phosphatidylinositol 3-kinase/protein kinase B signal transduction by mediating phosphorylation of 'Ser-473' of protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), promoting their activation. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect mechanism. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. Also regulates the cGAS-STING pathway by catalyzing phosphorylation of CGAS, thereby impairing CGAS oligomerization and activation. Also regulates the cGAS-STING pathway by mediating phosphorylation of PARP1. |
| Protein Name | Dna-Dependent Protein Kinase Catalytic SubunitDna-Pk Catalytic SubunitDna-PkcsDnpk1Ser-473 KinaseS473kP460 |
| Database Links | Reactome: R-HSA-1834949Reactome: R-HSA-3270619Reactome: R-HSA-5693571Reactome: R-HSA-8866654 |
| Cellular Localisation | NucleusNucleolusCytoplasmCytosol |
| Alternative Protein Names | Dna-Dependent Protein Kinase Catalytic Subunit proteinDna-Pk Catalytic Subunit proteinDna-Pkcs proteinDnpk1 proteinSer-473 Kinase proteinS473k proteinP460 proteinPRKDC proteinHYRC proteinHYRC1 protein |
Information sourced from Uniprot.org