Human PRAME (Preferentially Expressed Antigen In Melanoma) Sandwich ELISA Kit (STJE0011636)
SPECIFICATIONS
General Information
| Short Description | This PRAME Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.105ng/mL |
| Detection Limit | 0.312-20ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | PRAME |
| Gene ID | 23532 |
| Uniprot ID | PRAME_HUMAN |
| Sample Type | tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed in testis. Detected in samples of kidney, brain and skin. |
| Post Translational Modifications | |
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation. The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding. In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation. Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. |
| Protein Name | Melanoma Antigen Preferentially Expressed In TumorsOpa-Interacting Protein 4Oip-4Preferentially Expressed Antigen Of Melanoma |
| Database Links | |
| Cellular Localisation | NucleusChromosomeCytoplasmGolgi ApparatusCell MembraneAssociates With ChromatinSpecifically Enriched At Transcriptionally Active Promoters That Are Also Bound By Nuclear Transcription Factor Y (Composed Of NfyaNfyb And Nfyc) And At EnhancersRecruited To The Golgi Apparatus In Response To Interferon Gamma (Ifng) Treatment |
| Alternative ELISA Names | Melanoma Antigen Preferentially Expressed In Tumors ELISA kitOpa-Interacting Protein 4 ELISA kitOip-4 ELISA kitPreferentially Expressed Antigen Of Melanoma ELISA kitPRAME ELISA kitMAPE ELISA kitOIP4 ELISA kit |
| output |
Information sourced from Uniprot.org