Human PR3 (Proteinase 3) Sandwich ELISA Kit (STJE0003363)

Name: Human PR3 (Proteinase 3) Sandwich ELISA Kit (STJE0003363)
SKU: STJE0003363
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0003363

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General Information

Short Description	This PR3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	PRTN3
Gene ID	5657
Uniprot ID	PRTN3_HUMAN
Specificity	This kit recognizes Human PR3 in samples. No significant cross-reactivity or interference between Human PR3 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Function	Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. Plays a role in neutrophil transendothelial migration, probably when associated with CD177. Triggers inflammatory processes in neutrophils by interacting with ADGRG3 upstream of F2RL1/PAR2 activation.
Protein Name	Myeloblastin Agp7 C-Anca Antigen Leukocyte Proteinase 3 Pr-3 Pr3 Neutrophil Proteinase 4 Np-4 P29 Wegener Autoantigen
Database Links	Reactome: R-HSA-140875 Reactome: R-HSA-449836 Reactome: R-HSA-6798695 Reactome: R-HSA-6803157
Cellular Localisation	Cytoplasmic Granule Secreted Cell Membrane Peripheral Membrane Protein Extracellular Side Membrane Raft Localizes Predominantly To Azurophil Granules (Primary Secretory Granules) In Neutrophils Secreted Upon Neutrophil Stimulation By Tnf-Alpha Lipopolysaccharide (Lps) Fmlp And Cxcl8/Il8 Or During Neutrophil Transmigration Following Secretion Tethered To The Cell Membrane By Cd177
Alternative ELISA Names	Myeloblastin ELISA kit Agp7 ELISA kit C-Anca Antigen ELISA kit Leukocyte Proteinase 3 ELISA kit Pr-3 ELISA kit Pr3 ELISA kit Neutrophil Proteinase 4 ELISA kit Np-4 ELISA kit P29 ELISA kit Wegener Autoantigen ELISA kit PRTN3 ELISA kit MBN ELISA kit
Specificity	This kit recognizes Human PR3 in samples. No significant cross-reactivity or interference between Human PR3 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-112	102
EDTA plasma (n=8)	93-108	100
Cell culture media (n=8)	95-109	100

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.47	1.34	4.12	0.51	1.40	4.05
Standard deviation	0.02	0.06	0.18	0.03	0.08	0.15
CV (%)	4.26	4.48	4.37	5.88	5.71	3.70

Citations

View product citations for antibody STJE0003363 on CiteAb