Human POLB protein (Recombinant) (N-His) (STJP008254)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human POLB-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | POLB |
| Gene ID | 5423 |
| Uniprot ID | DPOLB_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asn12-Glu335 |
Additional Info
| Function | Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase).through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase. |
| Protein Name | Dna Polymerase Beta5'-Deoxyribose-Phosphate Lyase5'-Drp LyaseAp Lyase |
| Database Links | Reactome: R-HSA-110362Reactome: R-HSA-110373Reactome: R-HSA-110381Reactome: R-HSA-5649702Reactome: R-HSA-5651801Reactome: R-HSA-5689880Reactome: R-HSA-73930 |
| Cellular Localisation | NucleusCytoplasmCytoplasmic In Normal ConditionsTranslocates To The Nucleus Following Dna Damage |
| Alternative Protein Names | Dna Polymerase Beta protein5'-Deoxyribose-Phosphate Lyase protein5'-Drp Lyase proteinAp Lyase proteinPOLB protein |
Information sourced from Uniprot.org