Human pNF-H (Phosphorylated Neurofilament H) Sandwich ELISA Kit (STJE0003913)

Name: Human pNF-H (Phosphorylated Neurofilament H) Sandwich ELISA Kit (STJE0003913)
SKU: STJE0003913
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0003913

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Short Description	This pNF-H Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	46.88pg/mL
Detection Limit	78.13~5000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	NEFH
Gene ID	4744
Uniprot ID	NFH_HUMAN
Specificity	This kit recognizes Human pNF-H in samples. No significant cross-reactivity or interference between Human pNF-H and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name	Neurofilament Heavy Polypeptide Nf-H 200 Kda Neurofilament Protein Neurofilament Triplet H Protein
Cellular Localisation	Cytoplasm Cytoskeleton Cell Projection Axon
Alternative ELISA Names	Neurofilament Heavy Polypeptide ELISA kit Nf-H ELISA kit 200 Kda Neurofilament Protein ELISA kit Neurofilament Triplet H Protein ELISA kit NEFH ELISA kit KIAA0845 ELISA kit NFH ELISA kit
Specificity	This kit recognizes Human pNF-H in samples. No significant cross-reactivity or interference between Human pNF-H and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	248.23	475.34	1765.40	238.06	485.70	1771.88
Standard deviation	16.46	26.71	62.50	15.28	20.35	78.32
CV (%)	6.63	5.62	3.54	6.42	4.19	4.42