Human PLIN2 (Perilipin-2) Sandwich ELISA Kit (STJE0002099)

Name: Human PLIN2 (Perilipin-2) Sandwich ELISA Kit (STJE0002099)
SKU: STJE0002099
Availability: InStock

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SPECIFICATIONS

STJE0002099

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Short Description	This PLIN2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	PLIN2
Gene ID	123
Uniprot ID	PLIN2_HUMAN
Specificity	This kit recognizes Human ADRP in samples. No significant cross-reactivity or interference between Human ADRP and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	Acylated.primarily with C14, C16 and C18 fatty acids. Phosphorylation at Tyr-232 by isoform 1 of CHKA (CHKalpha2) promotes dissociation from lipid droplets: dissociation is followed by recruitment of autophagosome machinery to lipid droplets and subsequent lipid droplet lipolysis.
Function	Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.
Protein Name	Perilipin-2 Adipophilin Adipose Differentiation-Related Protein Adrp
Database Links	Reactome: R-HSA-1989781 Reactome: R-HSA-9613354 Reactome: R-HSA-9613829 Reactome: R-HSA-9615710
Cellular Localisation	Membrane Peripheral Membrane Protein Lipid Droplet
Alternative ELISA Names	Perilipin-2 ELISA kit Adipophilin ELISA kit Adipose Differentiation-Related Protein ELISA kit Adrp ELISA kit PLIN2 ELISA kit ADFP ELISA kit
Specificity	This kit recognizes Human ADRP in samples. No significant cross-reactivity or interference between Human ADRP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.53	1.51	4.21	0.57	1.41	4.21
Standard deviation	0.03	0.07	0.22	0.03	0.08	0.14
CV (%)	5.66	4.64	5.23	5.26	5.67	3.33