Human PLAU (Urokinase-type plasminogen activator) CLIA Kit for Quantitative Detection

Name: Human PLAU (Urokinase-type plasminogen activator) CLIA Kit for Quantitative Detection
SKU: STJC0000783
Availability: InStock

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SPECIFICATIONS

STJC0000783

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Short Description	This human PLAU kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	PLAU
Gene ID	5328
Uniprot ID	UROK_HUMAN
Specificity	This kit recognizes Human PLAU/uPA in samples. No significant cross-reactivity or interference between Human PLAU/uPA and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Expressed in the prostate gland and prostate cancers.
Post Translational Modifications	Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. Produced as an inactive single-chain protein (pro-uPA or sc-uPA), is processed into the active disulfide-linked two-chain form of PLAU/uPA by a proteolytic event mediated, at least, by TMPRSS4.
Function	Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
Protein Name	Urokinase-Type Plasminogen Activator U-Plasminogen Activator Upa Cleaved Into - Urokinase-Type Plasminogen Activator Long Chain A - Urokinase-Type Plasminogen Activator Short Chain A - Urokinase-Type Plasminogen Activator Chain B
Database Links	Reactome: R-HSA-6798695 Reactome: R-HSA-75205
Cellular Localisation	Secreted
Alternative CLIA Names	Urokinase-Type Plasminogen Activator CLIA kit U-Plasminogen Activator CLIA kit Upa Cleaved Into - Urokinase-Type Plasminogen Activator Long Chain A - Urokinase-Type Plasminogen Activator Short Chain A - Urokinase-Type Plasminogen Activator Chain B CLIA kit PLAU CLIA kit
Specificity	This kit recognizes Human PLAU/uPA in samples. No significant cross-reactivity or interference between Human PLAU/uPA and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	50.71	153.72	344.70	47.96	144.02	334.79
Standard deviation	4.23	13.60	30.13	6.20	12.80	22.67
CV (%)	8.34	8.85	8.74	12.93	8.89	6.77